Digital Circuits And Logic Design Samuel C Lee Pdf Download
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Our work shows that the power of synthetic biology lies in the large-scale design of new biological devices, allowing the rational construction of novel digital circuits that can be used to engineer cellular regulatory networks14,15.
More generally, these results show that the development of genetic parts with useful properties can be efficiently used to enable new digital logic. Going forward, we believe that existing parts9,10,11 will be used to develop increasingly more complex circuits that will be used in a range of applications, from understanding cell behaviour to engineering therapeutically useful cells.
However, the ability to build these circuits in living cells also comes with many challenges. In particular, the assembly of large DNA circuits to achieve complex, digital logic can be difficult and time consuming. For this reason, the demonstration that such a task can be performed in living cells has important implications. The technology described here can be used to assemble a library of DNA parts that are all present in equal amounts in yeast cells. This library can then be screened for functional parts with simple, basic electronic properties such as digital ON and OFF states. This approach can be applied to any type of DNA part, including a diverse set of protein parts. Digital parts that can be made in living cells have many applications.
From the cell’s perspective, digital circuits may be easiest to study because they can be switched on and off by the addition or removal of a single input, which can be much simpler than the often complicated effects that complex, analogue circuits can have. Our results show that it is possible to obtain digital circuits in living cells, and that these circuits can be used to build larger circuits that have the ability to sense and respond to environmental input.
Future versions of this work may include the development of comprehensive libraries of parts that can be easily combined into high-precision circuits that could be used to build systems that mimic the complexity of natural genetic circuits.
The use of DNA to program synthetic decision-making circuits has an advantage over many existing technologies based on proteins. DNA is stable, easily synthesized and easily amenable to a variety of high-throughput screening methods.
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